摘要
用钌红染色法在电镜下观察普通小麦3B-2、天兰冰草(Agropyron intermdeium)和八倍体小冰麦中,接种大麦黄矮病毒后细胞表面糖蛋白的变化,发现抗性不同的材料在大麦黄矮病毒感染后其细胞表面的糖蛋白层变化趋势不同。对大麦黄矮病毒免疫的天兰冰草接种后看不到细胞表面的糖蛋白层有明显变化。高抗大麦黄矮病毒的八倍体小冰麦中,在病毒感染后多数细胞表面糖蛋白层明显加厚.而在没有抗性的普通小麦3B-2中,大麦黄矮病毒感染后细胞表面糖蛋白层无明显的加厚现象,多数细胞的糖蛋白层部分脱落或完全消失。我们推测,细胞表面的糖蛋白与植物对大麦黄矮病毒的抗性有密切关系。感染大麦黄矮病后细胞表面糖蛋白层的加厚现象可能是植物对病毒的一种抗性反应。
Changes in the cell surface glycoproteins in common wheat 3B-2, Agropyron intermedium and octoploid wheat-wheatgrass Zhong 5 after the inoculation with barley yellow dwarf virus (BYDV) were sdudied using electron microscopy and ruthenium red staining. The resul's indicated that, after the inoculation with BYDV, different changes in cell surface glycoproteins were observed in the plant species with different levels of resistance. In A. intermedium which is immune to BYDV, inoculation with BYDV did not cause significant change in cell surface glycoprotein layer. In cotoploid wheat-whcatgrass Zhong 5 which is highly resistent to BYDV, BYDV infection caused significant thickening in most cell surface glycoprotein layer.In common wheat 3B-2 which is susceptible to BYDV, BYDV infection did not cause thickening in cell surface glycoprotein layer, but in most cells, glycoproteins on the cell surface were partially peeled off or disappeared completely. Therefore, it is suggested that the glycoproteins on cell surface play certain roles in BYDV resistance. The phenomenon of the thickening of cell surface glycoprotein layer caused by BYDV infection was possibly a resistant ceaction to the virus.
关键词
小麦
冰草
小冰麦
大麦黄矮病毒
Common wheat
Agropyron intermedium
Octoploid wheat-wheatgrass
Barley yellow dwarf virus
Cell surface glvcoprotein
Resistance