摘要
PEG(聚乙二醇)是一种水溶性蜡。近年来,越来越多的人将该物质用于电子显微镜、免疫细胞化学等方面的分析研究。Basman等(1981)用于动物组织免疫电镜的研究,Kondo等(1981)用于光镜、扫描电镜、透射电镜相关性的研究,Wolosewick(1980)用于动物组织深层细胞的扫描电镜观察。本研究将PEG用于植物深层组织及细胞结构的观察,得到良好效果。
After being fixed, plant leaves were embedded in polyethylene glycol (PEG, molecular weight 4000) and sectioned 5—10μm in thickness. PEG in the slices was dissolved during the routine process and the slices were observed under Hitachi S-450 scanning electron microscope By using this method, it is easy to study the space structures of organelles such as chloroplasts, mitochondria, nucleus, nucleolus, etc. as well as their space inter relations.
