摘要
以软枣猕猴桃(Actinidia arguta Planch)叶片为试材,以MS为基本培养基,附加生长素(2.4-D、NAA)和玉米素(ZT)成功地诱导出愈伤组织,结果表明:软枣猕猴桃叶片在MS^+ZT 1mg/1+2.4-D 3mg/1的情况下能诱导出适合于建立悬浮细胞系的愈伤组织。并研究了愈伤组织生长年龄、激素浓度、肌醇和蔗糖浓度四个方面对建立悬浮细胞系的影响。表明愈伤组织以生长10天时接种活细胞率最高;以MS培养基附加ZT1mg/1+2.4-D3mg/1较好;肌醇以200mg/1圆形细胞频率最高;蔗糖以5％最适。
The leaves of Actinidia arguta Planch were cultured on MS basic med-ium supplemented with 2.4-D or NAA and zeatin in vitro. The callus wereinduced successfullg. The results have been obtained as follows: the callusfitting for the establishment of cell line coulb be obtained from leaves of Act-inidia arguta Planch on MS+ZT1mg/l+2.4-D3mg/l. Tbe highest cell surv-ival rate has been obtained from callus that had been cultured for ten days.The satisfied result have beenobtained on MS + ZT1mg + 2.4 - D3mg/l.The high-est frequency of round cell is medium with 200mg/l myo-inositol. Suitableconcentration of sucrose is 5%
出处
《植物研究》
CAS
CSCD
北大核心
1991年第3期77-83,共7页
Bulletin of Botanical Research
