异硫氰酸酯法固定化醇脱氢酶乳酸脱氢酶和辅酶Ⅰ

Immobilization of Alcohol Dehydrogenase, Lactate Dehydrogenase and NAD on Isothiocyanate Activated Support

本文探索了一条较为简便、实用的制备异硫氰酸酯活性载体的新方法,研究了用异硫氰酸酯共价法固定化醇脱氨酶、乳酸脱氢酶和辅酶Ⅰ的条件,并对三者进行了共固定化。按本实验方法得到的固定化醇脱氢酶和乳酸脱氢酶的活力回收可达8.9%和11.9%,得到的固定化三元全酶系统的辅酶再生能力为相应游离酶系统的4倍。

A new practical method of preparing activated agarose support by isothiocyanate process has been developed. Alcohol dehydrogenase, lactate dehydrogenase and NAD were immobilized separately and coimmobilized site-to-site on isothiocyanate activated support. The enzyme activity yields of the immobilized alcohol dehydrogenase and lactate dehydrogenase were 9% and 12% respectively. The regeneration ability of the NAD with the coimmobilized tribasic enzyme system obteined here was four times as high as that with the corresponding free enzyme system.