哈茨木霉 C184菌株对喀麦隆香蕉及大蕉根部病原菌的体外拮抗作用 (英文 )

Antagonism in vitro of Trichoderma harzianum C184 against the root pathogens of banana and plantain in Cameroon

体外测定了新近分离自喀麦隆香蕉及大蕉田间的哈茨木霉 (Trichoderma harzianum)菌株C184对香蕉和大蕉根部病原菌柱枝孢 (Cylindrocladium pteridis)及茄镰孢 (Fusarium solani) ,尖镰孢(Fusarium oxysporum)和曲霉 (Aspergillus sp.)的拮抗作用。在对峙培养中上述病原菌菌丝生长均受到明显抑制 ;在赛咯酚分层培养中木霉菌株对上述病原菌菌丝生长的抑制率为 6 7%～ 88% ;木霉菌株培养滤液对上述病原菌菌丝生长的抑制率为 5 2 %～ 87%。电镜下观察到木霉菌株对柱枝孢及镰孢霉的寄生过程 ,认为这可能是主要拮抗机制之一 ;此外木霉菌株在液体培养中产生的抗生素及细胞壁降解酶也可能是抑制病原菌菌丝生长的主要原因。

The Trichoderma harzianum strain C184 isolated recently from banana and plantain plantation in Cameroon was tested for its antagonism in vitro against Cylindrocladium pteridis (the causal agent of root necrosis on banana and plantain); Fusarium solani , Fusarium oxysporum and Aspergillus sp (secondary colonizers of root systems of banana and plantain) On dual culture the mycelial growth of the pathogens was significantly suppressed; on the filter culture that was inhibited with 67% 88%; the culture filtrate of the Trichoderma strain reduced the mycelial growth of the pathogen with 52% 87% Results showed that the mycoparasitism of the Trichoderma strain on the hypha of pathogens was one of the mechanisms of the inhibition of the pathogens Some cell wall lytic enzymes and antibiotics produced in the liquid culture might also involve in the mechanism of antagonism