马铃薯试管苗及微型种薯形成的几个因素研究

Study on factors involved in formation of in vitro potato seedlings and microtuber.

以马铃薯东农 30 3(Solanum tuberosum L.Dongnong30 3)的茎尖为外植体 ,研究了灭菌方式对茎尖成活率、BA与 2 ,4 - D不同浓度组合对诱导不定芽、光强对微型种薯形成以及催芽方式对发芽率的影响 .结果表明 ,70 %酒精 30 s,0 .1% Hg Cl2 1min组合茎尖成活率最高 ;2 mg/ L2 ,4 - D和 0 .4 mg/ LBA组合最利于诱导不定芽 ;弱光环境有利于试管薯的形成 ;试管薯经针刺后浸泡于 0 .5 mg/ L2 ,4 - D和 2 mg/ L赤霉素混合液 8h的处理 。

The effects of sterilization methods and various combinations of BA and 2,4 D in different concentrations on induction of adventitious buds, of light condition on microtuber formation, and of method of forcing on rate of bud sprouting in potato were studied. The results indicated that the sterilization using 70% ethanol 30 sec, 0.1% HgCl 2 1 min improved survival rate of the stem meristem; the combination of 0.4 mg/L BA and 2 mg/L 2,4 D enhanced adventitious bud induction; low supplemental light increased microtuber yield; thorned microtuber that experienced soaking in a solution of 0.5 mg/L 2,4 D and GA 3 for 8 hours gave the best rate of bud sprouting.