碘乙酰胺和罗丹明对不结球白菜子叶原生质体线粒体失活效果的影响

Effects of IOA and R-6G on Mitochondria Inactivation of Cotyledon Protoplast of Non-heading Chinese Cabbage

以 91H秋 - 2 1(矮脚黄 )不结球白菜为试材 ,研究了碘乙酰胺 (IOA)和罗丹明 (R 6G)对子叶原生质体线粒体失活效果的影响。结果表明 ,0 .5mmol·L-1的IOA ,在 2 5℃条件下处理 2 0min(分 ) ,就能将植板率从 15 .0 %降到 6 .6 % ;1.0mmol·L-1的IOA ,在 2 5℃条件下处理 10min(分 )可使不结球白菜子叶原生质体失活。 40 μg·mL-1的R 6G ,在 2 5℃条件下处理 30min(分 ) ,可有效地抑制不结球白菜子叶原生质体的分裂和愈伤组织的形成。利用R 6G使原生质体线粒体失活 。

The report studied the effects of IOA and R-6G on mitochondria inactivation of cotyledon protoplast of non-heading Chinese cabbage 91 HQiu -21 (Aijiaohuang). The results were as follows:0.5mmol·L -1 IOA could reduce the plating efficiency from 15.0% to 6.6% at 25℃ for 20min. While 1.0mmol·L -1 IOA would make all protoplasts inactivated at 25℃ for 10min. 40μg·mL -1 R-6G can effectively inhibit the protoplast division and calli formation at 25℃ for 30min,so inactivated mitochondria with R-6G eases the asymmetric cell fusion.