骨髓细胞门静脉输注诱导移植肾免疫耐受的临床研究初探

Preliminary clinical study on the induction of renal allograft tolerance by injecting the donor marrow cells through portal vein

目的 探索供者骨髓细胞受者门静脉输注诱导移植肾免疫耐受的临床应用方法。方法 8例亲属供肾肾移植受者 (实验组 ) ,移植术前经门静脉注射供者的骨髓细胞 ,进行移植后的临床效果观察 ,移植肾肾内免疫状态分析 ,尿细胞流式细胞分析和细胞因子测定 ,结果与 6例亲属供肾肾移植病人 (对照组 )进行比较。结果 实验组在临床上和移植肾免疫状态上均表现出免疫低反应性 ,IL- 10 (Interleukin- 10 )表达较高 ,TNF- a(Tumor necrosis factor- α)表达较低。结论 我们认为 ,将供者骨髓细胞进行受者的门静脉输注是一种可行的诱导免疫耐受的方法 。

Objective To induce donor specific renal graft tolerance by injecting the donor marrow cells through portal vein Methods The donor marrow cells was injected into 8 living related renal allograft recipients through portal vein The transplant outcome,intragraft immunocompetent state,urine flow cytometry and some cytokines were analysed and compared to control group(n=7) Results In experimental group,the immuno hyporectivity was expressed in clinic and intragraft immunocompetent state,IL 10(Interleukin 10)were significantly increased and TNF α(Tumor necrosis factor α)decreased(P<0 05) Conclusion It was believed that the intraportal injection of donor marrow cells could induce recipients specific to lerance to renal allograft