免疫印渍技术用于斯氏并殖吸虫病抗原检测的研究

Study on the Serodiagnosis of Paragonimiasis skrjabini by immunoblotting technique

采用免疫印渍技术分析了斯氏并殖吸虫 30、60和 90d虫体抗原组份与斯氏、卫氏并殖吸虫病人、华支睾吸虫、血吸虫、囊虫、包虫病人和健康人血清反应情况。结果发现斯氏和卫氏并殖吸虫病人血清均可识别 9条抗原多肽 ,分子量分别为 1 7、2 7、2 9、35 5、37 5、60、72和 90kDa ,其中 35 5、37 5和 39kDa三条抗原带可 1 0 0 %同 2种并殖吸虫病人血清起反应 ,特异性达 1 0 0 %。 72和 90kDa抗原多肽可与其他寄生虫病人和正常人血清发生交叉反应。不同虫龄期的抗原在反应条带上无明显区别 ,但以 30d龄虫体抗原的显色更清晰。结果提示 35 5、37 5、和

Total antigens were prepared from different stages, 30?60 and 90d of Paragonimus skrjabini for the immunoblot analysis probing with the sera from patients with Paragonimiasis skrjabini, Paragonimiasis westermani, other parasitic diseases, and healthy adults. The results showed that nine bands with the molecular weight of 17, 27, 29, 35 5, 37 5, 39, 60, 72 and 90kDa reacted with both types of paragonimiasis antisera, especially three antigenic bands of 35 5,37 5 and 39 kDa were found to give a consistent reaction with paragonimiasis antisera. Only the 72 and 90 kDa bands were also reacted with the antisera from other mentioned diseases and normal individuals. There was no marked difference among the antigenic components of 3 developing stages of the worms, but the antigens of 30d worms showed a stronger reaction in the immunoblot assay. The results suggested that the 35 5,37 5 and 39 kDa components were the sensitive and specific antigenic marker for the diagnosis of human Paragonimiasis skrjabini and Paragonimiasis westermani.