摘要
目的 获得细粒棘球蚴脂肪酸结合蛋白 (FABP)基因序列资料 ,并进行序列分析。方法 从包虫包囊内获取细粒棘球蚴原头蚴 (protocloex) ,提取RNA和DNA ,分别以cDNA和基因组DNA为模板扩增出FABP基因 ;并将其分别克隆到T载体后进行序列测定和分析 ;同时将从cDNA扩增得到的FABP基因亚克隆到原核表达载体 (pTGEX - 4T)。结果 获得长度分别为 40 2bp和 482bp两个FABP基因 ,序列分析表明内含子区域在 349…42 7bp,同源性比较结果FABP基因ORF内一个碱基突变。结论 获得了FABP基因 ,为研究其免疫效果奠定了基础。
Objective To clone the FABP gene of Echinococcus granulosus and compare the nucleotide and amino acid sequence of FABP of E. granulosus between different isolates. Method RNA and DNA were prepared from the protocloex of the E.granulosus.FABP cDNA or gene fragment of E.granulosus was amplified from the E.granulosus cDNA or genomic DNA using specific primers. The PCR fragments were cloned into the T vector. The amplified cDNA fragment was also cloned into the pGEX 4T vector.Results Two fragments were amplified and cloned. Sequence analysis of the 482bp fragment revealed that the intron was located at 349~427. The cDNA fragment was 402bp.Conclusion The FABP gene was isolated and cloned from the hydatid tapeworm E. granulosus.
出处
《热带医学杂志》
CAS
2002年第2期143-146,共4页
Journal of Tropical Medicine