流体的不同流动方式对血管内皮细胞血管细胞粘附分子-1、核因子κB表达的影响

Influence of different flow pattern on VCAM-1, NF-κB expression in vascular endothelial cells

目的 :探讨在低切应力时不同流态对粘附分子表达的影响。方法 :置人脐静脉内皮细胞单层于平板流动腔内 ,经切应力 0 2Pa层流或振荡流作用 4h、18h ,用组织免疫化学方法测其血管细胞粘附因子 - 1(VCAM - 1)表达及核因子 (NF -κB)核转位活性 ,原位杂交法检测VCAM - 1mRNA表达。结果 :在相同的低切应力作用下 ,层流组未见VCAM - 1mRNA、VCAM - 1有明显变化 ,而振荡流组则明显上调VCAM - 1mRNA、VCAM - 1表达分别为对照组 2倍、2 4倍及NF -κB核转位活性明显高于对照。结论 :VEC在低切应力作用下 ,振荡流明显上调VCAM - 1表达 。

AIM:[WTBZtive of this study was to examine the influence of different flow pattern, particularly low shear stress flow regimens, on VCAM-1 and NF-κB in vascular endothelial cells. [WTHZ] METHODS: HUVEC covered sheets were placed within rectangular parallel plate flow chambers. Cells were subjected to 4, 18 h of either laminar or oscillatory shear stress. The expression of VCAM-1 and NF-κB activity were analyzed by immunohistochemistry. VCAM-1 mRNA was also detected using in situ hybridization. RESULTS: VCAM-1 mRNA and VCAM-1 expression were not upregulated by laminar shear stress. However, oscillatory shear stress significantly upregulated VCAM-1 mRNA and VCAM-1 expression. Translocation of NF-κB P65 was remarkably increased. CONCLUSION: In same low shear stress, the different expression of VCAM-1 mRNA and VCAM-1 were induced by HUVEC exposed to laminar or oscillatory flow patterns.