摘要
目的 :探讨烧伤血清对肠上皮细胞膜的损伤的机制及意义。方法 :大鼠肠上皮细胞株IEC - 6培养 ;烧伤血清刺激培养的IEC - 6后通过高效毛细管电泳分析、荧光偏振等技术 ,动态观察肠上皮细胞膜受作用后的变化。结果 :肠上皮细胞经烧伤血清作用后早期 ,细胞膜流动性下降 ,脂双层分子排序有序性增加 ,分子运动减少 ;同时肠上皮细胞膜的磷脂含量降低 ,组成成分发生改变 ,PLA2 活性增高。结论 :烧伤后肠上皮细胞细胞膜磷脂降解 ,膜流动性降低 ,这一系列变化互为因果从而导致肠上皮细胞膜结构和功能的损害 。
AIM: To explore the mechanism and significance of the intestinal epithelial cellular membrane damage following burn serum. METHODS: The intestinal epithelial cell(IEC-6) were cultured. The changes of total membranous phospholipid contents fluidity of the IEC membrane were dynamically examined with fluorescence polarization technique and HPCE. RESULTS: In the early stage after stimulation by 20% burn serum, the membranous fluidity obviously decreased. The total phospholipid contents decreased, the content of PLA 2 markedly increased. CONCLUSION: The serial changes in IEC after burned could result in the damages of IEC membrane structure, the integrity of cell membrane and function.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第7期790-793,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目 (No .392 90 70 0 -0 2 )
军队"九五"指令性课题 (96L0 4 2 )
