中性粒细胞对洗涤血小板聚集功能的影响

Effect of polymorphonuclear leukocytes on washed platelet aggregation

目的 :观察非激活或激活的中性粒细胞 (PMN)对洗涤血小板聚集功能的影响。方法 :采用Born方法测定血小板聚集功能。结果 :非激活的PMN( 5× 1 0 9cells/L)上清液对ADP和花生四烯酸 (AA)诱导的血小板聚集具有显著抑制作用 ,阿司匹林可增强这种抑制作用 ;肉豆寇佛波醇 (fMLP)及血小板活化因子 (PAF)激活的PMN悬液或其上清液均能活化血小板聚集 ,且PMN悬液的诱聚作用比其上清液更强 ;阿司匹林对fMLP或PAF激活的PMN悬液或其上清液均无明显抑制作用。结论 :不同状态 (非激活或激活状态 )的PMN对正常血小板的反应性表现出完全相反的作用 ,即非激活的PMN抑制血小板反应性 。

AIM: To investigate the effect of non-activated or activated polymorphonuclear leukocytes(PMN) on washed platelet aggregation. METHODS: Born's method was used to determine platelet aggregation.RESULTS: non-activated PMN (5×10 9 cells/L) significantly suppressed washed platelet aggregation induced by ADP or arachidonic acid. Aspirin enhanced this inhibition. N-formyl-methiongl-leucy-phenylalanine (fMLP)-or platelet-activating factor (PAF)-stimulated PMN strongly induced platelet aggregation, and the induction effect of PMN suspension was more active than that of PMN supernatant. Aspirin had no significant inhibitory effect on platelet aggregation induced by fMLP-or PAF-activated PMN. CONCLUSIONS: Different conditions of PMN (activated or non-activated) had the nearly opposite action on normal platelet reactivity. Briefly, non-activated-PMN inhibited platelet reactivity, whereas activated PMN stimulated it.