梗阻性黄疸大鼠的肾缺血再灌注损伤

Study on renal ischemic reperfusion injury in rats with obstructive jauncice

目的 研究短暂性肾缺血再灌注过程对梗阻性黄疸大鼠肾功能的影响 ,并观察脱氧胆酸钠在此模型中的作用。方法 实验大鼠分为正常对照组 (S组 )、正常肾缺血再灌注组 (SRI组 )、梗阻性黄疸组 (J组 )、梗阻性黄疸肾缺血再灌注组 (JRI组 )及梗阻性黄疸脱氧胆酸钠处理肾缺血再灌注组 (JTRI组 )。在胆道梗阻 1周后行双侧肾动脉夹闭 1 0min后放开 ,观察再灌注后 0、4、1 2、2 4h肝肾功能、内毒素水平及肾组织丙二醛、超氧化物歧化酶含量的变化。结果 JRI组内生肌酐清除率随再灌注时间延长呈持续性下降 ,JTRI组内生肌酐清除率的下降无明显缓解 ,J组、JRI组及JTRI组肾组织丙二醛水平明显升高 ,同时超氧化物歧化酶含量下降。结论 梗阻性黄疸状态下大鼠肾脏对缺血再灌注损伤的敏感性明显升高 ,缺血再灌注损伤可能与梗阻性黄疸术后急性肾功能衰竭的发生有关 。

Objective To explore the influence of temporary ischemic reperfusion (RI) on renal function and the curative effect of sodium deoxycholate on the model of jaundiced rats established by bile duct ligation. Methods Wistar rats were divided into 5 groups, including sham operation group (S group), sham operation and renal RI group (SRI group), common bile duct ligation group(J group), common bile duct ligation and renal RI group(JRI group), jaundiced rats treated with sodium deoxycholate and renal RI group(JTRI group). One week after bile duct ligation, rat bilateral renal arteries were transiently occluded for 10 min. Changes of renal histology and renal function were observed at different time points after renal reperfusion. Simultaneously, the contents of malondealdehyde (MDA) and superoxide dismutase 3 (SOD3) in the kidney were measured. Results Creatinine clearance rate Ccr was decreased progressively in both JRI and JTRI groups along with the prolongation of the duration of reperfusion. In J, JRI and JTIR groups, the level of MDA in the kidney tissue increased while SOD decreased. Conclusion Jaundiced rat is sensitive to RI injury. RI may be an important mechanism of acute renal failure after obstructive jaundice surgery. Sodium deoxycholate has no effects on this process.