单克隆抗体法检测尿中血红蛋白临床应用初探

Clinical application of a monoclonal antibody method in determining urine hemoglobin

目的 应用单克隆抗体法检测尿中血红蛋白 ,探讨鉴别尿中血红蛋白的准确而简便的方法 ,为临床提供可靠的实验依据。方法 测定单克隆抗体法及邻联苯胺法检测血红蛋白的灵敏度 ;应用单克隆抗体法及邻联苯胺法测定 30例健康者及 1 0 5例患者尿液。结果 单克隆抗体法检测尿血红蛋白最低检出浓度为 0 2 1 μg/ml,邻联苯胺法为 1 66μg/ml。 30例健康者尿液单克隆抗体法及邻联苯胺法检测结果均为阴性。1 0 5例患者尿液单克隆抗体法检测结果 97例阳性 ,8例阴性 ;邻联苯胺法检测结果 49例阳性 ,56例阴性 ,二者有显著性差异 (P <0 0 1 )。结论 单克隆抗体法检测尿血红蛋白特异性强 ,灵敏度高 ,作为鉴别尿中有无血红蛋白的准确而简便的方法 。

Objective To obtain an efficient method for determing hemoglobin in urine for clinical usage. Methods Monoclonal antibody method and benzidine method were employed to measure the urine hemoglobin of 30 normal individuals and 105 patients with erythrocytes >10/μl. The accuracies of the 2 methods were compared. Results The lowest detectable concentration of monoclonal antibody method was 0.2 μg/ml and that of benzidine method 1.66 μg/ml. The 30 samples of normal volunteers were negative with both methods. Among 105 patients 97 patients had positive result detected by monoclonal antibody method and 8 negative. While, benzidine method only found 49 cases were positive and 56 cases negative. Conclusion Monoclonal antibody method is accurate and specific and is suitable for determining urine hemoglobin in clinical work.