SSR标记用于玉米自交系遗传变异与优势类群划分的研究(英文)

Assessment on genetic relationships among 15 maize (Zea mays) elite inbred lines of China using SSR markers:comparisons with data from heterosis and pedigree

采用 SSR和杂种优势聚类方法分析我国 1 5个玉米骨干自交系的遗传变异 ,并初步进行了杂种优势类群划分。从 62个 SSR引物中筛选出的 40对有效引物对 1 5个玉米自交系进行了分析 ,共检测到 1 88个等位基因变异 ,每个 SSR座位的等位基因数 2～ 9个 ,平均为 4.7个。 SSR位点的多态信息含量 PIC值平均为 0 .675 ,分布范围在 0 .360～ 0 .85 1之间。根据SSR数据对供试材料进行遗传相似性分析 ,Nei氏相似性系数分布在 0 .5 74～ 0 .777之间。 1 0对多态性高的 SSR引物可有效区分 1 5个自交系。应用 SSR聚类分析的结果与系谱关系相一致 ,与杂种优势聚类法相比较 ,SSR方法具有效率高、结果可靠、可标准化的特点。对

Simple sequence repeats (SSR) was employed to investigate the genetic variation among 15 maize elite inbred lines in China.Forty SSR primers selected from all sixty two primers gave stable profiles amplified in the sample of 15 inbred lines,which could clearly resolve on 4% METAPHOR agarose gel.The average number of alleles per SSR locus was 4.7 with a range from 2 to 9.The polymorphism information content (PIC) for the SSR loci varied from 0.360 to 0.851 with a mean of 0.674 5.A total of 188 alleles identified served as raw data for estimating genetic similarities among these lines ranged from 0.574 between the combination of BJ8 vs.Jing7H up to 0.777 between HZ4 vs.4Z4.Six SSR primers,which had high level of polymorphism,could allow a rapid and efficient identification of 15 inbreds.A cluster diagram based upon the SSR data grouped the 15 lines into families consistent with the heterotic response of these.Comparing with heterosis grouping,SSR grouping is a more efficient,accurate and component measure.