转基因杨树的抗盐性分析

Analysis of Salt Resistance on the Poplar Transferred with Salt Tolerance Gene

以转 1 磷酸甘露糖醇脱氢酶基因的八里庄杨为试材 ,对获得的杨树转化体进行了不同NaCl梯度的组织培养、水培和盆栽试验。抗盐试验中 ,转基因八里庄杨比对照的始分化天、分化率、芽头密度、苗高、生长势、生根率明显得到提高 ;主根数、侧根数、根长的发生数量均较多。结果表明在 4‰含盐量的基质上 ,转基因苗木比对照有更好的抗性。

Poplar( Pupulus×Xiao zhannica,cv.“balizhuang-yang”) transferred with mtl-D gene was used as an experimental material. Through tissue culture, aquaculture and pot culture transgenic poplars are tested with variant Nacl salt gradient. In it transgenic poplar raises its initial days for differentiation, differentiation rate, bud intensity, bud height and growth potential than control plant. At the same time, the transgenic poplar has higher rooting rate and more top roots, side roots and higher root length than control under the same salt stress. The result shows that the transgenic poplar has better tolerance ability than control plant in the salt 4‰ contensity mediums.