转基因烟草检测技术研究

Study on detective technique for transgenic tobacco

本研究建立了转基因烟草常用调控基因35S启动子和nos终止子、标记基因npt-Ⅱ和目的基因TMV-CP的PCR检测技术,并用经构建的pUC18质粒提纯的35S启动子和nos终止子基因经地高辛标记后制成了基因探针,建立了检测35S启动子和nos终止子的分子杂交技术。杂交试验证明,PCR扩增的35S启动子和nos终止子为特异性扩增产物。此项检测技术具有快速、特异、敏感、经济以及可重复性强等优点。

In this research PCR detecting method for transgenic tobacco was established.35S promoter,nos termina-tor,marker gene npt-Ⅱand target gene TMV-CP was chosen as target templates for amplification.Then we have successfully accomplished probing detection by using constructed pUC18plasmid as templates for35S and nos.The results show that amplified products for35S promoter and nos terminator are specific by hybridization.All above in-dicate that the method we established was fast,sensitive,economic,reproductive and specific.