溶藻弧菌的间接荧光抗体快速检测

STUDIES ON THE RAPID DETECTION OF Vibrio alginolyticus BY INDIRECT FLUORESCENT ANTIBODY STAINING

建立了大黄鱼病原溶藻弧菌的间接荧光抗体检测方法。溶藻弧菌的特异性抗血清由新西兰兔制备 ,试管凝集法测定抗血清及交叉反应效价 ,吸附离心法去除交叉反应。荧光抗体为异硫氰酸荧光素标记的羊抗兔免疫球蛋白。现埸检测对具典型弧菌病表症大黄鱼病原检出率为100% ,表观健康大黄鱼病原检出率为30% ,养殖海水检测结果均为阴性。结果表明 ,间接荧光抗体检测法不仅可以用于已感染发病大黄鱼的快速检测 ,而且还能用于已感染未发病大黄鱼的检测。

A rapid detection of Vibrio alginolyticus infecting the Large yellow croaker Pseudosciaena crocea by indirect fluorescent antibody staining has been developed. The specific antisera for V. alginolyticus was prepared with New Zealand rabbit,and cross reactive efficacy was tested by coagulation in tube. It seemed that the goat anti rabbit IgG had been marked by fluorescent isothiocyanate (FITC). The results showed that the positive reaction detected in situ from the typical symptom P. crocea infected by Vibrio was 100%,30% from the health ones also suffered by this pathogen , but negative detection from its aquaculture sea water. It demonstrated that the rapid detection by indirect fluorescent antibody could be not only used for testing the ill yellow croaker infected by pathogen Vibrio, but also for those healthy ones with little pathogen.