青春双歧杆菌增菌培养基的优化

The Optimization of Reproducible Medium for Bifidobacterium adolescent

针对青春双歧杆菌的营养需要 ,采用正交实验优化基础培养基及增殖因子的用量。得出最佳基础培养基配比是 :大豆蛋白胨 1 2 % ,胰蛋白胨 0 6 % ,酪蛋白水解物 0 6 % ,牛肉浸膏 0 6 % ,葡萄糖 1 % ,低聚糖 0 5 % ,乳糖 0 5 % ,酵母浸出粉 0 7%。最佳增殖因子添加量是 :胡萝卜汁 1 5 % ,番茄汁 5 % ,肝浸液 1 5 % ,胱氨酸 0 0 5 %。最后使用优化出的增菌培养基配比测定生长曲线 ,同时测定 pH和滴定酸度的变化 ,确定增菌培养终止时间为 1 2h ,通过平板菌落计数 ,此时菌数可达 2 9× 1 0 9cfu/mL。

According to the nutritional needs of B adolescent, based culture and promoting reproduction factors were optimized by quadratic design The optimum based culture was as following:1 2% soya peptone, 0 6% tryptone, 0 6% casein enzymatic hydrolysate, 0 6% beef extract, 1% glucose, 0 5% oligofructose, 0 5% lactose, 0 7% yeast extract The optimum promoting reproduction factors were as following:15% carrot juice, 5% tomato juice, 15% liver infusion, 0 05% cystine Growth curve with optimized reproducible medium were determined, with the change of pH and titratable acidity According to the growth curve, the terminal time was at 12 hours B adolescent got to 2 9×10 9?cfu/mL in this culture medium for 12 hours at 37℃ under anaerobic condition