MICA基因与系统性红斑狼疮关系的研究

A study of the relationship between MICA gene and systemic lupus erythematosus

目的 明确云南汉族群体中主要组织相容性复合物 类链相关基因 A(majorhistocompatibility complex class I chian- related gene,MICA )基因与系统性红斑狼疮 (systemic lupuserythematosus,SL E)的关系。方法 应用 STR扫描分型技术、聚合酶链反应 -单链构象多态性和 DNA双向序列测定 ,确定 MICA基因第 4、5外显子在云南汉族 70例 SL E患者和 15 2名正常对照中的等位基因及其分布频率。结果 共发现 MICA第 5外显子 5种等位基因和第 4外显子 10种等位基因 ,并确定了各自在病例组和对照组中的分布频率。表明 MICA第 4、5外显子各等位基因的分布频率在 SL E病例组和对照组中差异无显著性。结论 云南汉族群体中 MICA基因第 4。

Objective To investigate the relationship between major histocompatibility complex class Ⅰ chain related A(MICA) gene and systemic lupus erythematosus (SLE).Methods The alleles and frequencies of exons 4 and 5 of MICA gene were determined in 70 cases of SLE and 152 controls of Yunnan Hans by STR genotyping, polymerase chain reaction, single strand conformation polymorphism and bidirection DNA sequencing. Results Five alleles of exon 5 and 10 alleles of exon 4 were found in this study. The frequency of each allele was determined in patients and controls. There was no significant difference between the two groups in exons 4 and 5 of MICA gene. Conclusion Exons 4 and 5 of MICA were not related to SLE in Yunnan Hans.