深二度烧伤后创面成纤维细胞的表型变化及意义

The significance of phenotypic regulation of wound fibroblasts on scar formation after deep partial-thickness burn injury

目的 研究深二度烧伤创面成纤维细胞的表型变化 ,探讨其与瘢痕形成的关系。方法 采用人皮肤深二度烧伤创面的系列组织标本 ,通过病理学、免疫组织化学、电镜学等手段 ,探讨创面成纤维细胞表达增殖细胞抗原 (PCNA)、α1(I)前胶原蛋白、α 平滑肌肌动蛋白的规律及透射电镜下超微结构的变化。结果 提示烧伤后创面成纤维细胞受激活 ,在不同时期向增殖表型、合成表型或收缩表型转化 ,透射电镜检查证实了这些演变规律。结论 烧伤后创面成纤维细胞的表型转化规律揭示了创面愈合过程瘢痕形成的部分规律性 ,值得进一步研究创面成纤维细胞的生物学特征与瘢痕形成的关系 ,为临床采用生物学方法防治瘢痕增生或挛缩提供理论依据。

Objective To investigate the relationship between phenotypic regulation of burn wound fibroblasts and the mechanism of scar formation.Methods A series of human wound tissues of deep partial thickness burn was taken to evaluate the expression of proliferating cell nuclear antigen(PCNA),α1(I) procollagen and α smooth muscule actin and to examine its ultrastructure under transmission electronic microscope.Results Wound fibroblast,activated after burn injury,undertook the phenotypic properties of proliferation,synthesis of collagen and contraction,which could be confirmed by the observations under transmission electronic microscope.Conclusion Our findings revealed to some extent the biological and molecular mechanism of scar formation in human after burn.The behaviors of wound fibroblasts are worthy of further studies,which might provide some theoretical basis for clinically decreasing hypertrophic scarring and/or scar contracture in deep partial thickness burn wound healing.