视网膜色素变性发病过程中αA-晶体蛋白基因转录的变化

Transcription Changes of αA-crystallins Gene during the Development of Retinal Degeneration in Rd, Rds and C3H Mouse

目的:确定 retinal degeneration（rd）、Ytinal degeneration slow（ls）和 C3H/J’鼠视网膜aA-晶体蛋白与其视网膜变性发病的关系。方法:本文在不同视网膜色素变性（Retinitis piglnentosa,RP）病程进展时期（出生后3、4、5、6、8w）,提取了三种ar动物模型小鼠a、ras和oH,以及正常对照小鼠＊s视网膜全mnnx,用逆转录聚合酶链反应（Reverse transcription-polymerase。chain reaction,RTPCR）获得了视网膜全cDNA样品;以 β-actin为内对照的 PCR（Polymerase chain reaction）,半定量测定了它们视网膜中 αA-晶体蛋白的表达。结果:随着视网膜变性病程的进展,αA-晶体蛋白mRNA的表达并没有变化,这与RCS（Royalcollege of sutgeons）大鼠的表达减少有明显不同。结论:mJ 和 C3H小鼠视网膜变性进程与αA-晶体蛋白的表达无关,其视网膜变性的分子机制可能与RCS大鼠不同。

Purpose: To determine the relationship between retinal αA-crystallins and the development of retinal degeneration in rd, rds and C3H mice.Methods:Total retinal mRNA was prepared from the retina of (rd)retinal degeneration, (rds)retinal degeneration slow, C3H and control C3B mice during the progress of retinal degeneration (Post-natal 3, 4, 5, 6, 8 weeks). The retinal expression of αA-crystallins was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis.Results: Expression of retinal αA-crystallins is rather stable in rd, rds and C3H mice during the progress of their retinal degeneration. The expression is also similar to that in normal C3B mice but different to that in Royal college of surgeons (RCS) rats. Conclusion: Retinal αA-crystallins may not involve in the course of RP in rd, rds and C3H mice. The molecular mechanism of RP in rd, rds and C3H may be different to that in RCS rat.