胃癌中α-及β-连接素联合表达

Immunohistochemical Coexpression of α-catenin and β-catenin in Human Gastric Cancers

目的 :探讨α 及β 连接素 (catenin)联合表达与胃癌侵袭转移间的关系。方法 :应用ABC染色法在同一胃癌术后标本上进行α 及β catenin抗体染色 ,计数胃癌组织中阳性细胞所占的比例。 结果 :正常胃黏膜上皮细胞全部表达α 及 β catenin。 38例胃癌组织中α 及 β catenin阳性表达率分别为2 8 9% (11/ 38)和 5 0 % (19/ 38)。存在淋巴结转移的胃癌其α 及 β catenin的阳性表达率分别为 8 7%及 2 6 1% ,均远低于无淋巴结转移者 (P <0 0 1)。α 与 β catenin共同阳性表达占 30 % ,共同阴性表达占 36 9%。α catenin(- ) / β catenin(- )表达者最易发生淋巴结转移 ,α catenin(+ ) / β catenin(- )表达者易发生肝转移。结论 :检测α ,β

Objective To research the relationship between coexpression of α ,β catenin and invasion and metastasis of gastric cancer.Methods To perform ABC method to immunostain for α and β catenin antibody in the same postoperation samples of gastric cancers,then to number the proportion of positive cells.Results Both of these catenins were expressed strongly in all of the normal gastric epithelial cells.The positive expression rate of α and β catenin in 38 cases of gastric cancers was 28 9%(11/38) and 50%(19/38) respectively.In cancers with lymph node metastasis,the positive expression of α and β catenin was 8 7%(2/23) and 26 1%(6/23),which was significantly lower than in node negative tumors (P<0 01).The common positive and negative expression of these two catenins was 30% and 36 9% respectively.Lymph node metastasis is easy to occur in the cancers with α catenin(-)β-catenin(-)expression,whereas liver metastasis is the most common in the cancers with α catenin(+)β catenin(-)expression.Conclusions The detection of coexpression may be more exact and sensitive at predicting invasion and metastasis of gastric cancer.