VEGF基因表达与肝细胞癌生物学特性的研究

Study on Relationship Between Expression of VEGF and Biology Characters in Hepatocellular Carcinoma

目的 :研究血管内皮生长因子 (vascularendothelialgrowthfactor ,VEGF)蛋白及mRNA与肝细胞癌生物学特性的关系。方法 :应用逆转录 聚合酶链反应 (RT PCR)及ABC免疫组化染色技术 ,检测 6 0例肝细胞癌中VEGFmRNA及蛋白表达情况 ,并分析它们与肝细胞癌临床及病理的关系。结果 :VEGFmRNA及蛋白的阳性表达率分别为 78 3%和 81 3% ,明显高于正常肝组织 (P <0 0 5 ) ;并且VEGF表达与肿瘤的病理分级、有无包膜、瘤栓、直径大小均有相关性 ;另外 ,VEGFmRNA和蛋白表达呈正相关关系。结论 :VEGFmRNA和蛋白表达水平越高 ,细胞恶性程度及侵袭、转移能力越强 。

Objective To study the relationship between expression of VEGF mRNA,protein and clinicopathology in hepatocellular carcinoma.Methods The expression of VEGF mRNA and protein in 60 patients with hepatocellular carcinoma were detected by RT PCR and immunohistochemistry (ABC method) and the relationship between expression of VEGF and its clinicopathology was evaluated.Results The positive rates of VEGF mRNA,protein were 78 3%,81 3% in tumor tissues and they were significantly higher than that in normal liver tissue P <0 05).The expression of VEGF was correlated with the histologic grade and metastasis of tumor.The expression of VEGF mRNA and protein was closely positively related.Conclusions The results showed that the higher expression of VEGF mRNA and protein was associated with the higher malignant degree and more chances of metastasis in hepatocellular carcinoma. VEGF plays an important role in growth of tumor.