乳腺癌早期外周血淋巴细胞亚群FCM检测分析

Analysis of Lymphocyte Subpopulations of Peripheral Blood for Patients with Breast Cancer by FCM

目的 :探讨乳腺癌早期肿块大小及有无腋淋巴结转移对免疫功能状态的影响及临床意义。方法 :手术前 3～ 6d抽取外周静脉血 ,FCM检测CD+3总T淋巴细胞、CD+4 辅助T细胞、CD+8杀伤 /抑制T细胞、CD+4 /CD+8比值、CD+56 NK细胞、CD+19总B淋巴细胞、CD+2 5白介素 2 (IL 2 )受体细胞比例。结果 :乳腺癌T2 N1M0 组较T2 M0 M0 组CD+4 辅助T细胞显著增高 ,其他淋巴细胞无显著性变化 ;T1N0 1M0 和T2 N0 1M0 两组间、T1N0 M0 和T2 N0 M0 两组间比较 ,各淋巴细胞亚群均无显著性差异。结论

Objective To investigate weather the size of the mass and the axillary lymph node metastasis may influence the changes of the immunological function for patients with breast cancer.Methods To take the peripheral blood from 40 patients with breast cancer and detect the proportion of the CD + 3total T cells,CD + 4Th cells,CD + 25 IL 2 R cells,CD + 56 NK cells,CD + 8 Tc/Ts cells,CD + 19 total B lymphocyte with the series of the monoclonal antibody by FCM from 3 to 6 days before the operation.Results The proportion of CD + 4 Th cells in T 2N 1M 0 group increased significantly compared with that in T 2N 0M 0 group before the operation.The proportion of lymphocyte subpopulations showed no significant changes both between T 2N 0 1 M 0 and T 2N 0 1 M 0 groups and between T 1N 0M 0 and T 2N 0M 0 groups.Conclusions The size of the mass and the axillary lymph node metastasis have no obvious influence on immunological function for patients with breast cancer in early stages.