支原体肺炎一氧化氮与脂质过氧化水平的变化

Changes of Nitric Oxide and Lipid Peroxide in Patients with Mycoplasma Pneumoniae Pneumonia

为了探讨一氧化氮 (NO)、丙二醛 (MDA)在肺炎支原体肺炎不同时期的变化 ,应用生化法检测了正常组、肺炎支原体 (MP)感染组、肺炎支原体肺炎 (MPP)组患儿血清中 NO、 - OH、 MDA的含量 ,并应用免疫组化法检测了外周血单个核细胞 (PBMCs)中 i NOS的表达状况。结果表明 :1MP感染组、MPP组患儿血清 NO、 - OH及 MDA水平均明显高于正常对照组 (P<0 .0 1) ,MPP组又明显高于 MP感染组 (P<0 .0 1) ;2在 MPP组中 ,c Tn I阳性组患儿血清 NO、 - OH与 MDA水平较 c Tn I阴性组患儿明显增高 (P<0 .0 1) ;3在 MPP组中 ,c Tn I阳性组患儿 PBMCs中i NOS阳性细胞率明显高于 c Tn I阴性组 (P<0 .0 1)。提示 :NO介导的免疫损伤在 MPP的发生发展中起着重要的作用 ,并可能是 MPP继发心肌损伤的重要原因之一。

In order to investigate the changes of nitric oxide (NO) and malonydialdehyde (MDA) in patients with mycoplasma pneumoniae (MP) pneumonia, the contents of serum NO, -OH and MDA in the normal children (control group), the children with infection of MP (MP group), the children with MPP (MPP group) were determined by using the methods of biochemistry techniques and the expression of iNOS was detected by using immunohistichemistry methods. It was found: (1) As compared with control group, the contents of serum NO,-OH and MDA were higher in MP group and MPP group (both P <0 01); (2) In the MPP group , the contents of serum NO, -OH and MDA in the children positive for cTnI were higher than in the negative ( P <0 01); (3) In the MPP group, the positive rate of iNOS in PBMCs in the children positive for cTnI was higher than in the negative ( P <0 01). The results showed that NO mediated immune injury may play an important role in the pathogenesis of MPP and it seems to be an important factor for MPP following myocardial injury disease.