CD40分子在乳腺癌组织中表达的研究

Study on CD40 molecular expression in breast carcinoma

目的 探讨乳腺癌组织中CD40分子表达与癌组织浸润转移的关系 ,以及其与ER、PCNA之间的相关性。方法 运用免疫组织化二步法 (Envision法 )对 42例福尔马林固定石蜡包埋的乳腺癌组织进行回顾性研究 ,并且选择 1 5例纤维腺瘤患者作为对照。结果 CD40、ER和PCNA在乳腺癌组织中表达阳性率分别为 35 .7%、54 .8%和 47.6 % ,与纤维腺瘤组相比 ,具显著性差异 (P <0 .0 5或P <0 .0 1 ) ;ER、PCNA与淋巴结转移有关 (P <0 .0 5或P <0 .0 0 1 ) ,CD40与淋巴结转移无相关性 (P >0 .0 5) ;CD40在乳腺癌的表达与ER、PCNA并不存在显著相关性 (P >0 .0 5)。结论 细胞发生恶变后 ,其分子表达常常发生改变 ,从而导致细胞的生物学行为异常。CD40分子在乳腺癌组织中的异常表达可为乳腺癌的诊断。

Objective To explore the relation between CD40 molecular expression and metastasis in human breast carcinoma,and the correlation between expression of CD40,ER and PCNA.Methods Formalin fixed and paraffin embeded tissues from 42 cases of breast carcinoma were studied by Envision immunohistochemical technique,and 15 fibroadenomas as controls.Results The positive rate of CD40,ER and PCNA in 42 samples of human breast carcinoma was 35.7%,54.8% and 47.6%,and there were significant differences compared with that of fibroadenomas(P<0.05 or P<0.01).ER?PCNA in lymph node positive tissues were higher than those in lymph node negative tissues(P<0.05 or P<0.001),and CD40 expression wasn′t correlated with lymph node metastasis(P>0.05).The expression of CD40 wasn′t related to that of ER and PCNA(P>0.05).Conclusion After cells become malignant,its molecular expression often changes,and biological behavior of cells is abnormal.Abnormal expression of CD40 molecule may offer reliable evidence for diagnosis,treatment and prognosis in breast carcinoma.