原发性胆囊癌CD44v6表达的免疫组化研究

An immunohistochemical study on expression of CD44v6 protein in human gallbladder carcinoma

目的 研究CD44v6在胆囊癌中的表达及其临床意义。方法 应用免疫组织化学Elivision二步法测定 40例原发性胆囊癌和 1 2例慢性胆囊炎 ,6例胆囊腺瘤、4例胆囊腺肌症等良性病变CD44v6表达。结果 CD44v6在胆囊癌组织中阳性表达率为 72 .5 % ,胆囊良性病变阳性表达率为 0 % ,胆囊癌组织与胆囊良性病变CD44v6表达水平之间有显著性差异 (P <0 .0 1 ) ,且CD44v6表达与胆囊癌淋巴结转移、临床分期、分化程度有关。结论 CD44v6基因的表达 ,有望成为判断胆囊癌淋巴结转移。

Objective To investigate the clinical significance of CD44v6 protein expression in human gallbladder carcinoma(GBC).Methods The immunohistochemical technique was adopted for detecting the CD44v6 protein expression in 40 cases of GBC,12 cases of chronic cholecystitis,6 cases of adenoma and4 cases of adenomyomatous hyperplasia.Results The positvie rate of CD44v6 expression in gallbladder carcinoma and benign lesions was 72.5%,0% respectively(P<0.01),and it also correlated with the lymph node metastasis,pathologic differentiation and clinic staging,but had no correlation with pathological types.Conclusion Detecting CD44v6 expression might be severed as an objective indicator for differential diagnosis,lymph node metastatic potency,tumor progression and prognosis in gallbladder carcinoma.