等容血液稀释对大鼠局灶性脑缺血作用的研究

The effect of isovolemic hemodilution on focal cerebral ischemia in rats

以血液稀释治疗血栓栓塞性脑缺血为目的,采用大脑皮层局部血流量(rCBF)、脑组织含水总量以及脂质过氧化物含量(LPO)为重要指标,共71只大鼠的实验研究,结果表明:大脑局灶性缺血24小时后经血液稀释治疗2小时,血细胞压积由49.4±3.5%降至39.3±3.77%,rCBF增加19.95%(n=16,P<0.005),脑组织含水量及LPO含量,对照组与稀释组分别为81.39±1.56%(n=12),79.00±1.08%(n=13,P<0.001);645.57±106.72nmol/100mgpro(n=11),360.47±117.46nmol/100mgpro,(n=14,P<0.001)。提出血液稀释可增加rCBF,减轻脑水肿,降低LPO。

This study examines the effect of isovolemic hemodilution on cerebral ischemia of thromboembolism for 24 hours in 71 Wistar rats. The rats were randomly divided into a hemodilution and a control group. At the end of 2 hr hemodilution, the hematocrit was reduced from 49.4±3.5% to 39.3±3.7% (n=43)。The rCBF of the embolized hemisphere increased by 10.95 (n=16, P<0.05). The values of the brain water content and the LPO of brain tissue were 81.39±1.56% (n=12), 79.00±1.08% (n=13, P<0.001) and 645.57±106.72nmol/100mg pro. (n=11), 360.47±117.46nmol/100mg pro.(n=14, P<0.001) in the control and the hemodilution groups respectively. The results suggest that hemodilution may increase rCBF, decrease brain edema and linid nernxidation of brain tissue.