人外周血淋巴细胞对绵羊红细胞的破坏作用

Human peripheral blood lymphocytes causing destruction of sheep erythrocytes

人外周血淋巴细胞,在含Ca^(2+)、Mg^(2+)的Hanks液中与绵羊红细胞(SRBC)共育,能将SRBC破坏释出血红蛋白(Hb),半数溶血值为0.1921±0.06,经胰蛋白酶抑制后淋巴细胞破坏SRBC的能力明显减弱,半数溶血值为0.0348±0.03。经尼龙棉柱法及E-玫瑰花法把淋巴细胞分离为T、B和NK等各个亚群。引起溶血作用最强的是NK细胞。对同一血源标本,同时用^(125)IUdR释放法测定NK活性和溶血分光光度法测定溶血活性,其结果呈明显正相关。本文认为人外周血淋巴细胞有破坏绵羊红细胞的能力,有这种能力的淋巴细胞是NK细胞。可望用本实验的溶血分光光度法测定NK细胞的活性。

Human peripheral blood lymphocytes and sheep red blood cells(SRBC)were incubated together in Hanks solution containing Ca^(2+) and Mg^(2+) ion, causing SRBC destruction and release of hemoglobin, 500%hemolysis value was found to 6.1921±0.06. The destruction of SRBC by lymphocytes was inhibited by trypsinase, its 50% hemolysis value became 0.0348±0.03 (P<0.001). Lymphocytes were seperated into T, B and NK subpopulations by the nylon cotton fibre and E rosette test, NK cells showed the strongest hemolytic effect. Nk activity and hemolytic activity showed marked positive correlation in the same sample when measured simultaneously by ^(125)IUdR release method and hemolysis spectrophotometer method. It is considered that human peripheral blood lymphocytes have the ability to destroy SRBC, those lymphocytes which have such ability are NK cells. The activity of NK cells can be measured by the hemolysis spectrophotometer method.