多因素阿尔茨海默病动物模型的建立及针刺对其海马胆碱酯酶活性影响的实验研究

Observation of acupuncture on cholinesterase activity of hippocampus in multifactorial model of Alzheimer's Disease

目的 建立一个集衰老、铝中毒与胆碱能系统多因素损害的阿尔茨海默病 (AD)动物模型 ,研究针刺对其海马胆碱酯酶 (ChE)活性的影响。方法 以西药哈伯因作对照 ,通过海马ChE活性检测 ,验证针刺的作用效果。结果 发现在D 半乳糖亚急性衰老基础上的单纯铝中毒模型和单纯东莨菪碱 (SCOP)模型均显示出类似AD的海马胆碱酯酶活性改变 ,多因素方法较单因素方法有更明显的神经生化改变。结论 本研究提示多因素损伤方法可以更好地模拟AD的病因和发病机制的特征 ,并首次证明针刺有提高多因素AD模型动物海马ChE活性的作用 。

Objective To improve the method of inducing Alzheimer's disease(AD) model in rats, we attempted to construct a multifactorial AD animal model to examine the effect of acupuncture treating AD through cholinesterase (ChE) activity in the hippocampus. Methods Compared with Huperzine A, we selected D-galactose, scopolamine and aluminum toxicosis as the inducing agent to obtain the AD model to examine the effect of acupuncture. Results The model rats given D-galactose, scopolamine and aluminum toxicosis showed decreasing hippocampi ChE activity. The result indicated that multifactorial AD model had significant neurobiochemical changes more than single factor model. Conclusion The multifactorially damaged AD model mimics the etiologic and pathogenetic characteristics of dementia model of rats with learning and memory impairment. This is the first time to affirm that acupuncture can improve the hippocampal ChE activity, which offers the experimental proof for the validity of acupuncture treatment of AD.