丹参对心肌缺血和再灌注损伤的保护作用

The protective effects of redix salviae miltiorrhizae on the ischemic and post-ischemic reperfusion injury of the heart

采用在家兔全麻、开胸、自主呼吸和自主心律的条件下,结扎冠脉左室支造成急性心肌缺血模型,进而松开结扎结形成再灌注损伤模型。对心肌缺血和再灌注损伤的组织脂质过氧化物含量和局部血流量变化进行测定,同时辅以心电图监护;以丹参注射液为保护剂观察其作用效果。结果表明,随着缺血时间的延续,心肌脂质过氧化物含量逐渐增加;当缺血60分钟后再灌注30分钟,脂质过氧化物含量仍继续上升,明显高于缺血60分钟组,但与缺血90分钟组比较则无显著差异;其缺血区局部组织血流量再灌注后仅恢复53.2%。给予丹参保护的再灌注组,其缺血区组织脂质过氧化物含量较再灌注损伤组下降56.0%(P<0.005),而局部组织血流量恢复则提高32.0%(P<0.001)。

We studied the protective effects of redix salviae miltiorrhizae (RSM) on the ischemic and post-ischemic reperfusion injury which was made by ligating and reopening of the left ventricular branch of the coronary artery of the open-chest anaethetised rabbits under the supervision of ECG. There were 14 rabbits in each group, 7 of them for the measurement of myocardial lipid peroxide level (MLPL) by the thiobarbituric acid method (Hiroshi Ohkawa, 1979), and the other 7 for measuring distribution of regional myocardial blood flow (RMBF) by the biological radionuclidelabeled particle method (Wang Jingxian, 1985). With increasing duration of ischemia, MLPL increased significantly in the ischemic and post-ischemic reperfusion injured heart. In the case of 60 mins ischemia followed by 30 mins reperfusion, MLPL was much higher than that without reperfusion. However, which compared with that of 90 mins ischemia, no significant difference was found. The recovery of RMBF was only 53.2% after 30 mins of reperfusion following 60 mins of ischemia. The occurance of post-ischemic reperfusion injury was dependent on the duration and severity of ischemia. The injury of reperfusion after long period (60 mins) of ischemia is similar to that of increasing duration of ischemia. No-reflow phenomenon was observed in ischemic zones during reperfusion after 60 mins of ischemia. In the RSM (2g/kg body wt) protected reperfusion group. MLPL was reduced by 56.0% and the recovery of RMBF was increased by 32.0%.