石膏对家兔白细胞致热原性发热效应和脑脊液环核苷酸含量变化的影响

Effects of gypsum on both the fever and the cyclic nucleotids levels in CSF in rabbits with fever

本研究用白细胞致热原(leucocytic pyrogen,LP)复制家兔发热模型,观察石膏灌胃对家兔发热效应和脑脊液环核苷酸含量变化的影响。结果发现:60分钟时,石膏+LP组动物△T℃(0.40±0.08),TRI_3(2.55)明显低于LP组△T℃(0.91±0.06),TRI_3(4.99),分别比较两组△T℃和TRI_3,其差异均非常显著(P<0.01)。脑脊液cAMP含量:石膏+LP组(17.48±1.27pmol/ml)明显低于LP组(25.14+2.11pmol/ml),而cGMP含量在60分钟时石膏+LP组(17.40±1.36pmol/ml)则明显高于LP组(12.70±0.50pmol/ml),差异均非常显著(P<0.01和P<0.005)。作者推论:石膏煎剂灌胃对家兔发热效应有明显抑制作用,其机制很可能与中枢组织中环核苷酸含量变化有关。

The purpose of this study was to investigate the effects of gypsum on LP-induced fever and the CSF levels of cAMP and cGMP in rabbits. It was found that gypsum could inhibit the rise of body temperature caused by LP injection, (△T of gypsum +LP group is 0.40±0.08℃, TRI_3 2.55; LP group 0.91±0.06℃, TRI_3 4.99 P<0.01) and that cAMP levels in CSF could be decreased by gypsum, comparing with that of other groups. But a significant increase of cGMP levels in CSF was found in this experiment (cAMP levels of gypsum + LP group in CSF are 17.48±1.27 pmol/ml, LP group 25.14±2.11 pmol/ml, and cGMP levels of experiment animals 17.40±1.36 pmol/ml, LP group 12.70±0.50 pmol/ml. P<0.01 and 0.005). This shows that gypsum can inhibit LP-induced fever; changes of cyclic nucleotide levels in the central nervous system might play a major role in febrifugal mechanism of gypsum.