G6PD缺乏患者红细胞膜ATP酶活性研究

Study on Na^+-K^+ ATPase and Ca^(2+) -Mg^(2+) ATPase activities of human erythrocyte membrane in G6PD deficient individuals

本文采用低渗溶血,高速旋离沉淀法制得人红细胞膜制剂,再利用酶学比色法测定了11例正常人和11例G6PD缺乏者红细胞膜Na^+-K^+ATP酶及Ca^(2+)-Mg^(2+)ATP酶活性。结果发现G6PD缺乏时红胞细膜Ca^(2+)-Mg^(2+) ATP酶及Na^+-K^+ATP酶活性均有不同程度的降低。哇巴因抑制率下降49.4%。这些异常可能导致患者红细胞膜结构及红细胞的生理功能受损。

By using Person's separating blood ghost method basically, Na^+-K^+ ATP ase and Ca^(2+)-Mg^(2+) ATPase activties of human erythrocyte membrane were studied. The comparison was made between 11 G6PD deficient subjects and 11 healthy control persons. The results showed that both enzyme activities as well as ouabain inhibition rate were decreased in G6PD deficient individuals (P<0.01). The authors proposed that these changes may damage the membrane structure and function.