P物质对人单核细胞膜HLA-DR和HLA-DQ抗原表达的影响

Effect of substance P on the expression of HLA-DR and HLA-DQ antigens on human monocytes

将正常人外周血单核细胞(monocytes,Mon)与不同浓度的P物质(substance P,SP)共同温育24小时,借抗HLA-DR(Tü36,德国)和抗HLA-DQ(Tü22,德国)的单克隆抗体以碱性磷酸酶标记法(APAAP法),在光学显微镜下检测Mon表达HLA-DR和HLA-DQ抗原的情况。结果表明:高于血浆生理浓度的SP(10^(-10)mol/L,10^(-8)mol/L,10^(-6)mol/L)能明显地增加正常人外周血中HLA-DQ^+Mon的百分率(48.8±2.5%,54.5±2.6%,49.0±4.3%),与对照组者(37.4±1.7%)相比,分别为P<0.01,P<0.01,P<0.05,其中以10^(-8)mol/L SP的作用最强,而血浆生理浓度的SP(10^(-12)mol/L)则不能明显地增加HLA-DQ^+Mon的百分率(41.8±3.1%),与对照组者相比,P>0.05。在本实验条件下,上述浓度的SP对Mon膜HLA-DR抗原表达的影响不明显(P>0.05)。作者推测,在应激反应时,血中增多的SP可能借其对免疫反应的正调节作用而部分对消过多糖皮质激素的免疫抑制作用。

Recent studies have shown that the the nervous system possesses immunoregulatory function. We investigated the effect of substance P(SP) a neuropeptide on the expression of HLA-DR and HLA-DQ antigens on normal human monocytcs of the peripheral blood. APAAP (alkaline phosphatase-antialkalinc phosphatasc) enzyme immunoassay was adopted to detect the HLA class Ⅱ molccults, using monoclonal antibodies Tu36, Tu22, in the reaction system. The results, showed that SP at concentrations higher than the physiological serum level (10^(-10)mol/L, 30^(-8)mol/L, 10^(-6)mol/L) promoted the expression of HLA-DQ antigen on monocytcs significantly in vitro (P<0.01, P<0.01, P<0.05), while SP at the physiological serum concentration (10^(-12)mol/L) was not found to enhance the expression of HLA-DQ antigen(P>0.05). The maximal effect was observed at 10^(-8)mol/L. Expression of HLA-DR antigen, however, was not influenced by SP in the same range of SP concentrations. It is reasoned by the authors that the immunosupprcssivc effect of excessive glucocorricoids during stress might partly be counterbalanced by the uprcgulatory action of increased blood SP on the immune function.