幽门螺杆菌培养上清液对大鼠胃黏膜上皮细胞增殖的影响

Effect of Helicobacter pylori broth culture supernatant on cell proliferation in rat gastric mucosa

目的 :研究幽门螺杆菌 (Helicobacterpylori,Hp)的代谢产物在胃黏膜上皮细胞增殖中的作用。 方法 :用液体培养的方法培养产毒Hp菌株NCTC116 37,然后提取上清液 ,用浓缩上清液 (concentratedbrothculturesuper natant,CCS)灌鼠胃。采用放射免疫方法测定血请胃泌素 ,用免疫组织化学SP(streptavidin/ perosidase)法检测胃黏膜增殖细胞核抗原 (proliferationcellnuclearantigen ,PCNA)和表皮生长因子受体 (epidermalgrowthfactorreceptor,EGFR)的表达。结果 :灌服CCS组与对照组比较 ,胃黏膜PCNA标记指数阳性率升高、差异具有显著性 ,胃黏膜细胞表皮生长因子受体在灌服CCS组有 4/ 8阳性 ,对照组无 1例阳性 ;血清胃泌素升高 ,但统计学上差异无显著性。结论 :产毒Hp培养上清液可以促进胃黏膜上皮细胞的增殖 。

Objective: To study the effect of Helicobacter pylori broth culture supernatant on cell proliferation in rat gastric mucosa. Methods:NCTC11637 was cultured and its concentrated broth culture supernatant(CCS) prepared, then given to Wistar rats orally,and finally serum gastrin was measured by radioimmunoassay, and the expression of proliferation cell nuclear antigen(PCNA), and epidermal growth factor receptor(EGFR) in gastric mucosa were examined by immunohistochemical method. Results: The expressions of PCNA and EGFR were higher in the rats given CCS of Hp Conclusion: CCS of NCTC11637 Hp can promote the proliferation of rat gastric mucosal cells, and may have relation with promotion of gastric carcinogensis through increasing gastric cell proliferation.