摘要
观察仙贞片对糖尿病大鼠主动脉糖基化终产物受体mRNA高表达的影响。采用链脲佐菌素左下腹腔内单次注射造成糖尿病大鼠模型 ,随机分模型组、仙贞片组及氨基胍组各 10只 ,另设正常对照组 10只。应用原位杂交的方法。结果模型组大鼠主动脉内皮细胞糖基化终产物受体mR NA表达的灰度值明显增高 ,出现过度表达。仙贞片治疗后主动脉糖基化终产物受体mRNA表达的灰度值降低 ,与氨基胍组相近似 (P >0 0 5 ) ,与模型组比较差异显著 (P <0 0 5 )。显示仙贞片与氨基胍一样 ,可显著下调糖基化终产物受体 -mRNA的过度表达 ,对糖尿病大鼠主动脉血管糖基化终产物受体高表达有明显调节作用。提示仙贞片对高血糖造成的主动脉血管损害具有保护作用。
The effects of Xianzhen Tablets (XT) on the expression of RAGE mRNA in the aorta were observed in the diabetic rats. The diabetic rat model was induced by a single injection of streptozotocin into the left lower part of the abdominal cavity of the animal. The diabetic model rats were randomly divided into the model group, XT treating group, and aminoguanidine treating group, and there were 10 rats in each group. A normal control group consisting of 10 rats was also set up. The in situ hybridization method was adopted for the experiment. The results showed that the gray scale value of the expression of RAGE mRNA was markedly increased in endothelial cells of the aorta, manifesting over expression, in the model group; the gray scale value of the expression of RAGE mRNA decreased in endothelial cells of the aorta in the XT treating group, which was similar to that in the aminoguanidine treating group ( P >0 05), and very different from that in the model group ( P <0 05). The results suggest that as same as aminoguanidine, XT can decrease the over expression of RAGE mRNA, exerting a marked regulative effects on the increased expression of RAGE mRNA in the aortic vessel, which implies that XT has protective effects against the damage to the aortic vessel by hyperglycemia.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2002年第4期21-24,共4页
Journal of Beijing University of Traditional Chinese Medicine
关键词
仙贞片
糖尿病
大鼠
主动脉
mRNA
糖基化终产物受体
Diabetes
Aorta
Xianzhen Tablets
Receptor of Advanced Glycation End Products
RAGE mRNA