奥曲肽抑制人胃癌细胞株MKN45的生长

Somatostatin analog octreotide inhibited the growth of human gastric cancer cell MKN45 in vitro

目的 探讨奥曲肽 (Octreotide,OCT)对人胃癌细胞株MKN45生长的调控作用。方法 采用MTT比色分析法分别测定OCT对生长于无血清培养基及含 1 0 %胎牛血清 (FBS)培养基中MKN45细胞的调控作用。结果 OCT 1 0 -2 、1 0 -3 、1 0 -4 g L对培养于无血清培养基中的MKN45的生长均有抑制作用 ,以 1 0 -3 g L浓度的抑制作用最显著 ,为 1 0 .4% ;而OCT 1 0 -2 、1 0 -3 、1 0 -4 、1 0 -5、1 0 -6g L对生长于含 1 0 %FBS培养基中的MKN45均未显示出抑制作用。结论 OCT可抑制无血清培养基中的MKN45的生长。胎牛血清中可能含有可快速降解OCT的肽酶 ,使得OCT未显示出对生长于含 1 0 %FBS培养基中的MKN45的抑制作用。

Objective To study the effects that the somatostatin analog octreotide (OCT) exerted on the growth of human gastric cancer cell MKN45.?Method MTT colorimetric assay was used to measure the growth of MKN45 cell cultured in RPMI 1640 medium with and without 10% FBS, respectively.?Results 10 -2 ,10 -3 ,10 -4 g/L OCT inhibited the growth of MKN45 cell cultured in serum-free medium and the maximum inhibitory rate was 10.40% by 10 -3 g/L OCT; while OCT 10 -2 ,10 -3 ,10 -4 ,10 -5 ,10 -6 g/L did not exert any inhibitory activity on MKN45 grown in the medium containing 10% FBS.?Conclusions OCT inhibited the growth of human gastric cancer cell MKN45 cultured in serum-free medium. It is possible that there are peptidases in serum, which result in the rapid degradation of OCT.