奥曲肽对人胃癌细胞株抑制作用机制的实验研究

Mechanism of inhibition of human gastric cancer cell line growth in vitro by the somatostatin analog octreotide

目的 研究奥曲肽 (OCT)对人胃癌细胞株MKN45生长的调控机制。方法 采用流式细胞术分析OCT1 0 -3 g L对MKN45细胞的周期分布的影响。结果 1 0 -3 g L这一浓度可诱导MKN45出现G0 G1 阻滞 ,于加入OCT后 6h开始 [(63 .67± 3 .92 ) % ] ,2 4h最显著 [(75 .0 5± 3 .42 ) % ] (P <0 .0 5) ,36h已消失 ;与对照组 [(7.0 3±0 .54) % ]相比 ,2 4hG2 M期细胞比例亦减少 [(2 .41± 1 .97) % ] ,但OCT未改变亚二倍体细胞的比例。结论 OCT可抑制人胃癌细胞株MKN45的生长 ,其作用机制之一是诱导细胞出现G0 G1

Objective To study the effect and mechanism the somatostatin analog octreotide exerts on the growth of human gastric cancer cell MKN45. Methods MTT colorimetric assay and flow cytometric analysis of DNA histograms were used to measure the proliferation and cell cycle distribution of MKN45 cell. Results 10 -2 ,10 -3 ,and 10 -4 g/L OCT exerted inhibitory actions on MKN45 cells, and the maximum inhibitory rate was 10.4% at 10 -3 g/L OCT. OCT(10 -3 g/L) also induced MKN45 to G 0/G 1 arrest, which began[(63.67±3.92)%]6 h after OCT was added, reached the maximum at 24 h (75.05%± 3.42%), and then disappeared at 36 h. At 24 h, the percentage of G 2/M cells also decreased [(2.41±1.97)%], compared with the control[(7.03±0.54)%]. OCT did not change the percentage of hypodiploid cells. Conclusion Inhibition of the growth of human gastric cancer cell MKN45 in vitro by the somatostatin analog octreotide involves G 0/G 1 phase arrest.