交河故城古车师人的线粒体DNA分析

Analysis of Mitochondrial DNA from the Ancient Ruins of Jiao-he

从距今 2 0 0 0～ 2 5 0 0年左右的交河故城古代人骨中提取古 DNA,用 4对重叠引物对线粒体基因组的调控区 (3 63 bp)进行了扩增及测序 .线粒体基因组编码区的扩增片段用于限制性片段多样性分析 .结果显示4个个体中具有 3个 DNA序列 ,其中来自不同墓穴的两个个体的序列相同 ,说明这两者间有密切的母系遗传关系 .系统发育分析表明古车师的这 4个个体分散分布在现代新疆维吾尔人的序列之中 .从这些结果可以初步得出结论 ,即古车师人群并不是一个同源群体 ,在早期铁器时代 。

Mitochondrial DNA(MtDNA) was successfully extracted from four individual bones( femurs ) from 2 tombs at the ancient Ruins of Jiao he in Turfan basin, dated about 2000~2500 years ago. Through four overlapping primers, we got nucleotide sequence of 363 bp length. Ancient MtDNA was analyzed by restriction fragment length polymorphism(RFLP) analysis for the A, B, C and D haplogroups and the sequencing of hypervariable region Ⅰ of the MtDNA control region.The result shows that three haplotypes with 7 polymorphic sites in 4 individuals were obtained. Two specimens (male in each tomb) share the same haplotype. It is reasonable to assume that the close blood relationship existed between them. Phylogenetic analysis indicated that Jushi population were scattered among modern Xinjiang Uighuis and did not fall into specific clusters, so our preliminary data imply that Jushi population was not a genetically homogenous population and an ancient mingling of Euro Asian population had existed prior to the early iron age.