鼠疫F_1抗原的纯化及分子量测定的研究

STUDY ON PURIFICATION OF Y.PESTIS FI-ANTIGEN AND DETERMINATION MOLECULAR WEIGHT

提纯的鼠疫F_1—McAb与经环氧氯丙烷活化的琼脂糖凝胶Sepharose4B偶联,制备成F_1—McAb—Sepharose 4B亲和层析柱。将鼠疫F_1抗原粗品纯化后,经SDS—聚丙烯酰胺凝胶电泳检测,仅出现一条沉淀带,与标准蛋白分子量带比较测得鼠疫F_1抗原分子量为26kDls。

The purification Y. pestis F1-McAb were coupled with activation Sepharose-4B column by epichlohydrin. The chromatography affinity column of Y. pestis F1-McAb Sepharose-4B had been, preparated. After Y. pestis F1-antigen of rough products of market selling would be pass through the column. Then the pure products of Y. pestis F1-antigen was identified by sodium dodecly sulfate (SDS) polyacrylamight and only appeared the precipitation belt. It was compared with stardard prodein belt and Y. pestis F1-antigen molecular weight was identified for 4.316×10^(-23)kg(26k Dls).