高三尖杉酯碱诱导鼻咽癌细胞CNE-2Z凋亡过程中Caspase-6、-8的活性变化

Changes of the caspase-6 and -8 activity during the homoharringtonine-induced apoptosis in nasopharyngeal carcinoma cell CNE-2Z

目的 了解高三尖杉酯碱 (HHT)诱导CNE 2Z细胞凋亡过程中Caspase 6、 8的活性变化。方法 用不同浓度 (0 .12 5、0 .2 5、0 .5、1μg/ml)的HHT分别处理CNE 2Z细胞 1、2、4、8、12、2 4h ,用比色法检测其Caspase 6、 8的活性 (A40 5nm表示 )。结果 1μg/mlHHT处理CNE 2Z细胞 1h即可见Caspase 8活性明显高于两个对照组 (未处理组和抑制剂 +药物处理组 ,P <0 .0 1) ,2h达高峰。而Caspase 6的活性在 4h开始升高 ,12h达高峰 ,2 4h仍明显高于对照组 (P <0 .0 1)。不同浓度的HHT处理CNE 2Z细胞 ,Caspase 6在 12h、Caspase 8在 2h时均可见它们的活性明显高于两个对照组 (P <0 .0 1) ,且随HHT浓度的增加而活性增强。结论 Caspase 6、 8参与了HHT诱导的CNE 2Z细胞凋亡 ,且其活性升高有时间和浓度依赖性。

Objective To study the changes of the Caspase 6 and 8 activity during the homoharringtonine(HHT) induced apoptosis in nasopharyngeal carcinoma CNE 2Z cells. Methods The CNE 2Z cell line was treated with HHT at 0.125?0.25?0.5 ?1 μg/ml for 1?2?4?8?12?24h respectively. The Caspase 6 and 8 activities were detected by colorimetric assay ( A 405nm ). Results When CNE 2Z cell line was treated with 1μg/ml HHT,Caspase 8 activity increased at 1h and reached the peak at 2h, which were much higher than those of two control groups( non treated group and inhibitor + drug treated group, P <0.01). The Caspase 6 activity reached the peak at 12h and still significantly higher than that of two control groups. When the CNE 2Z was treated with different concentrations of HHT, Caspase 6 and 8 activities were obviously higher than those of control groups ( P <0.01).With the increasing concentration of HHT, the Caspase 6 and 8 activities also elevated. Conclusion Caspase 6 and 8 take part in the course of the HHT induced apoptosis of CNE 2Z cells. The increase of Caspase 6 and 8 activities is in a time and concentration dependent manner.