桂皮酸对急性早幼粒细胞白血病NB4细胞增殖和分化作用

Acute promyelocytic leukemia cell proliferation inhibition and differentiation induced by cinnamic acid

目的 探讨桂皮酸 (CinnamicacidCINN )对急性早幼粒细胞白血病 (APL)细胞增殖和分化的作用。方法 将APL细胞株NB4细胞与不同浓度CINN在体外共同培养 ,应用台盼蓝拒染试验及MTT比色法观察细胞生长曲线、生长抑制率 ;用光学显微镜观察细胞形态变化 ;以NBT还原试验检测药物作用前后NB4细胞NBT还原能力改变 ;用流式细胞仪测定细胞周期及细胞分化抗原CDlla、CDllb的表达。结果 lmmol/LCINN、3mmol/LCINN和 5mmol/LCINN能明显抑制NB4细胞增殖 ,呈剂量时间依赖性 ;lmmol/L、3mmol/LCINN能诱导NB4分化 ,表现在NBT还原能力显著增强 ,CDlla、CDllb表达升高 ,药物组与对照组之间差异有显著性 (P <0 .0 1) ,流式细胞术分析发现 ,CINN处理组细胞被阻滞于S期 ,S期细胞数明显增多 ,G2 期细胞数减少。结论 CINN体外能诱导NB4细胞分化 ,增殖能力下降。

Objectives To investigate APL cell proliferation inhibition and differcntiation induced by cinnamic acid. Methods In vitro,NB 4 cells as incubated and with CINN, the cell growth was tested by 3 (4,5 dimethy thiazol 2 yl) 2,5 diphenyl tetrazdium bromide (MTT) and trypan blue stain assay. The cell differentiation was observed by cytomorphology ,nitroblue tetrazolium (NBT) test. The cell cycle and CDlla, CDllb antigens, cell cycle were analysed by flow cytometry . Results In short period NB 4 cells were induced to morphologically and functionally more differentiated cells.Cell growth curve showed that growth of NB 4 cells were inhibited. NBT reduction was rearkedly increased and expression of CD1la and CDllb was also increased with significant statistical differences between the treated and control groups( P <0.01). Flow cytometry analysis showed that CINN arrested NB 4 cell in S phase and the number of cells in s phase was nedrkedly increased Conclusion CINN can induce differentiation and growth inhibition of NB 4 cells.