不同浓度的一氧化氮对食管癌细胞核DNA的损伤作用

DAMAGE EFFECT OF VARIOUS CONCENTRATION NITRIC OXIDE ON THE NUCLEAR DNA IN THE ESOPHYGEAL CARCINOMA CELLS

目的 :研究不同浓度的一氧化氮 (nitricoxide,NO)对食管癌细胞核DNA损伤作用的特征性规律。方法 :以硝普钠 (sodi umnitroprusside ,SNP)作为NO的体外供体 ,以食营癌细胞系EC10 9作为细胞模型 ,采用单细胞凝胶电泳法检测不同浓度的SNP作用一定时间后 ,食管癌细胞核DNA被损伤的情况。结果 :无论SNP的作用时间是 8h ,还是 16h ,彗星状细胞核所占的百分率均越来越高 ,经 χ2 检验差异有显著性意义 (P <0 .0 1)。曲线回归分析结果表明 ,食管癌细胞彗星状细胞核生成的百分率与SNP的浓度之间明显相关。结论 :在一定的条件下 。

Purpose: To study the effect of nitric oxide (NO) in various concentrations on the nuclear DNA damage in esophageal carcinoma cells. Methods: Sodium nitroprusside (SNP) was used as the donor of NO in vitro, and EC109 esophageal carcinoma cell line as the cell model. The damage effect of NO was examined using the single cell gel electrophoresis assay. Results: When SNP effected time periods were whether for 8 h or 16 h, the SNP concentrations were increased at each time point, percentages of the comet tail of nuclei were higher and higher, their difference was significant by χ\+2 test (P<0.01). Regression analysis indicated that the percentage of the comet tail in the esophageal carcinoma nuclei was highly correlated to the SNP concentration. Conclusion: The damage effect of NO on nuclear DNA depends on its concentration in esophageal carcinoma cells.