中华(上海)骨髓库北方人群HLA多态性调查

Investigation on the HLA Polymorphism of North China Population in Shanghai Bone Marrow Bank

调查中华(上海)骨髓库北方人群HLA多态性及其频率分布特征。用微量淋巴细胞毒试验检测11995名无关供者HLA-A、B抗原,并用PCR-SSP和反向PCR-SSOP技术对疑难标本进行复检。计算其中3 736名北方人群 HLA-A、B的抗原频率、基因频率和HLA-A、B位点单倍型频率、连锁不平衡参数。调查中共检出A位点抗原26种,B位点抗原54种,最常见HLA-A、B抗原包括A2,A11,A24,A30,A33,B13,B46,B51,B58,B60,B61,B62等。连锁不平衡参数大于0.0005的单倍型有40多种,最常见的单倍型有A2-B46,A30-B13,A33-B58等。结果显示中华(上海)骨髓库北方人群HLA多态性有自身特点,频率分布介于南、北汉族之间。

To investigate the HLA polymorphism of north China population in Shanghai bone marrow bank, the HLA-A and HLA-B antigens of 11 995 of unrelated donors were detected by micro-lymphocyte cytotoxicity test (MLT ) , and the ambiguous cases were re-checked by PCR-SSP and reverse PCR-SSOP. The frequencies of HLA-A and HLA-B antigens, the gene frequencies, haplotype frequencies of HLA-A and HLA-B loci and the linkage disequilibrium parameters were calculated in 3 736 of north China individuals in this bank. Altogether, 26 A locus antigens and 54 B locus antigens were detected, the most common HLA-A and HLA-B antigens included A2, Ali, A13, A30, A33, B13, B46, B51, B58, B60, 861, B62. There were 40 haplotypes with linkage desequibilibrium parameter more than 0.0005, the most common haplotypes were A2-B46, A30-B13, A33-B58. The results indicate that the HLA polymorphism of north China pop-ulations show its special characters, and the distribution of frequencies is between the south and north Han-population.