地方性氟中毒患者外周血淋巴细胞姐妹染色单体交换的研究

A study of sister chromatid exchange(SCE)in peripheral lymphocytes of patients with endemic fluorosis

用荧光加 Giemsa 技术,区分姐妹染色单体,分析92人外周血淋巴细胞的 SCE,比较地方性氟中毒患者和对照组 SCE 频率变化,发现53名氟中毒患者 SCE 频率(9.15±2.39)高于39名对照组(4.56±0.88),P<0.01。认为 SCE 增高是由高氟引起,氟是一种诱变因素。SCE频率的增高是高氟造成遗传物质损伤的证据之一。

With the Hoechst-33258 plus Giemsa Stain employed to distinguish the sisterchromatid exchange(SCE),the frequencies of SCE in the peripheral lymphocy-tes of 53 patients with endemic fluorosis were analyzed as compared with those of39 normal ones in control group.It was found that the SCE frequency of patientwith endemic fluorosis was significantly higher(9.15±2.39)than that in normalcontrol group(4.56±0.88,p<0.01),and highly suggested that fluoride may bea mutagenic factor.