亚硒酸钠对人血淋巴细胞合成DNA的影响

Effect of sodium selenite on DNA synthesis in human blood lymphocytes

微量法培养人外周新鲜全血和 PHA 刺激淋巴细胞转化,用~3H-TdR 参入 DNA 合成观测不同浓度亚硒酸钠对转化淋巴细胞增殖的影响。结果表明,低浓度亚硒酸钠(1.18×10-~7mol)促进细胞的增殖,细胞 DNA 合成比对照组平均增高43.5%,而高浓度亚硒酸钠(1.18×10-~5mol)抑制细胞的增殖,细胞 DNA 合成比对照组平均降低93.3%。本实验除表明不同浓度亚硒酸钠对细胞增殖的上述双相作用外,还用回归作图法求得此双相作用的临界亚硒酸钠浓度为1.0×10-~6mol。

Selenium effect on DNA synthesis in normal human peripheral bloodlymphocytes has been studied by radiometry method based on ~3H—TdR incorporatedDNA of inverted lymphocytes.The results showed that sodium selenite at lowdoses(1.18×10^(-7)mol)enhanced the cells proliferation whereas,at high doses(1.18×10^(-5)mol),it inhibited the cells proliferation,for the total DNAsynthesis,as measured by^3H—TdR incorporation in DNA,increased 43.5%and decreased 93.3%,respectively,in comparison with controls.The criticalvalue of the diphasic effect of sodium selenite was 1.0×10^(-6)mol.