摘要
目的 :探讨地黄不同品种 (种质 )的遗传多样性和亲缘关系 ,为地黄育种提供依据。方法 :采用 2 0个10bp随机引物对 19个地黄不同品种的基因组DNA进行RAPD分析。 结果 :共扩增出 16 3个位点 ,其中多态性位点 114条 ,占 6 9.9%。根据SPSS 10 .0分析软件中的Jaccard相似系数矩阵绘制遗传关系树系图 ,19个地黄品种分为北京系列、85 5系列、郭里茂等 4个类型。结论 :北京系列的 8个品种首先聚在一起 ,85 5系列中 5个品种较早地聚在一起 ,表明其类内具有较近的亲缘关系。这一关系为地黄的育种材料的选择提供了参考。
Objective:To provide molecular evidences for its breeding by studying the genetic relationship among varieties of Rehmannia glutinosa . Method:Nineteen varieties were detected by Randomly Amplified Polymorphic DNA(RAPD) markers. Result:The 20 selected primers produced 163 bands, among which 114(69.9%) were polymorphic. A DNA molecular dendrogram was established based on Hierarchical cluster analysis of 163 DNA bands amplified by 20 primers,which divided the 19 varieties into four groups:Group Beijing,Group 85 5,Group Guolimao and the other Group. Conclusion:8 varieties of Group Beijing have a close genetic relationship,and so have varieties of Group 85 5,which provides information for Rehmannia glutinosa 's breeding.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2002年第7期505-508,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金重点项目资助 ( 39930 2 2 0 )
关键词
地黄
RAPD
亲缘关系
品种
Rehmannia glutinosa
RAPD
genetic relationship
