人类染色体17p13.3位点新克隆基因HC56、HC71和HC90对Jurkat细胞活力的作用

Effects on viability of Jurkat cell of novel cloned gene HC56,HC71 and HC90 mapped at human chromosome 17p13.3

目的 探讨人类染色体 17p13.3位点新克隆基因HC5 6、HC71和HC90对T淋巴瘤细胞株Ju rkat细胞活力的作用。方法 应用脂质体介导的基因转染方法 ,将外源基因HC5 6、HC71和HC90分别导入Jurkat细胞 ,用苔盼兰拒染试验 ,观察基因的瞬时表达对细胞活力的作用。结果 Jurkat细胞瞬时转染HC5 6和HC71基因后 ,与转染PBK/CMV空载体的实验对照组比较 ,第 2 4、4 8、72和 96h ,细胞的活力明显受抑制 (P <0 .0 1) ;Jurkat细胞瞬时转染HC90基因后 ,仅在第 96h细胞的活力受抑制 (P <0 .0 5 ) ,而第 2 4、4 8和 72h ,未发现细胞的活力受抑制 (P >0 .0 5 )。结论 外源HC5

Objective To investigate the effects on cell viability of T lymphoma cell line Jurkat of HC56, HC71 and HC90 mapped at human chromosome 17p13.3. Methods HC56, HC71 and HC90 gene were transfected into Jurkat cells respectively by the medium of liposome. The effects on cell viability of the exogenous gene products were observed by trypan blue dye exclusion test. Results The viability of Jurkat cells at 24, 48, 72 and 96 hours after HC56 and HC71 gene transfected was inhibited significantly( P <0.01) as compared with control cells transfected with PBK/CMV empty vector.The viability of Jurkat cells at 24?48 and 72 hours except at 96 hours after HC90 gene transfected was not inhibited significantly( P >0.05). Conclusion Exogenous HC56 and HC71 gene products could inhibit viability of Jurkat cells.